human ctgf  (MedChemExpress)

Journal: Nature Communications

Article Title: Role of CTGF-LRP1 in impaired healing of cesarean section incisions

doi: 10.1038/s41467-026-69747-9

Figure Lengend Snippet: a Schematic of experimental groups: fibroblasts derived from Adjacent, Control, and Niche tissues were treated with recombinant human CTGF (rhCTGF). b Western blot analysis of Collagen I and III in fibroblasts following rhCTGF treatment. c Schematic of transcriptomic analysis in human uterine fibroblasts (HUFs) with modulated LRP1 expression under rhCTGF stimulation. d Collagen I and III expression in HUFs transfected with control siRNA (siNC) or LRP1 siRNA (siLRP1) and stimulated with rhCTGF. e Volcano plot showing differentially expressed genes (DEGs) between rhCTGF-treated siNC and siLRP1 HUFs. f GO analysis indicating suppression of ERK and WNT pathways in LRP1-silenced, rhCTGF-treated HUFs. g Western blot of phosphorylated ERK in siNC- or siLRP1-transfected HUFs treated with rhCTGF. h Phospho-ERK levels in Adjacent, Control, and Niche fibroblasts treated with rhCTGF. i Western blot analysis of Collagen I, Collagen III, and phospho-β-catenin in HUFs transfected with siNC or siLRP1 and treated with rhCTGF in combination with PD98059 (MEK1 inhibitor) or PAF-C16 (ERK1/2 activator). j Collagen I, Collagen III, and phospho-β-catenin expression in Adjacent, Control, and Niche fibroblasts treated with rhCTGF with or without PD98059 or PAF-C16. All in vitro experiments are performed in three independent replicates. Adjacent, adjacent myometrium tissue of niche; Control, well-healed cesarean scar tissue; Niche, niche tissue; HUF, human uterine fibroblasts; siNC, NC-siRNA; siLRP1, LRP1-siRNA. Source data are provided as a Source Data file. Created in BioRender. He, C. (2025) https://BioRender.com/krpej6u --.

Article Snippet: For functional assays, cells were treated for 48 hours with recombinant human CTGF (rhCTGF; HY- P70106 , MCE), PD98059 (MEK/ERK inhibitor, HY-12028, MCE), or C16-PAF (MEK/ERK agonist, HY-108635, MCE).

Techniques: Derivative Assay, Control, Recombinant, Western Blot, Expressing, Transfection, In Vitro

Journal: Nature Communications

Article Title: Role of CTGF-LRP1 in impaired healing of cesarean section incisions

doi: 10.1038/s41467-026-69747-9

Figure Lengend Snippet: a Schematic of experimental groups: fibroblasts derived from Adjacent, Control, and Niche tissues were treated with recombinant human CTGF (rhCTGF). b Western blot analysis of Collagen I and III in fibroblasts following rhCTGF treatment. c Schematic of transcriptomic analysis in human uterine fibroblasts (HUFs) with modulated LRP1 expression under rhCTGF stimulation. d Collagen I and III expression in HUFs transfected with control siRNA (siNC) or LRP1 siRNA (siLRP1) and stimulated with rhCTGF. e Volcano plot showing differentially expressed genes (DEGs) between rhCTGF-treated siNC and siLRP1 HUFs. f GO analysis indicating suppression of ERK and WNT pathways in LRP1-silenced, rhCTGF-treated HUFs. g Western blot of phosphorylated ERK in siNC- or siLRP1-transfected HUFs treated with rhCTGF. h Phospho-ERK levels in Adjacent, Control, and Niche fibroblasts treated with rhCTGF. i Western blot analysis of Collagen I, Collagen III, and phospho-β-catenin in HUFs transfected with siNC or siLRP1 and treated with rhCTGF in combination with PD98059 (MEK1 inhibitor) or PAF-C16 (ERK1/2 activator). j Collagen I, Collagen III, and phospho-β-catenin expression in Adjacent, Control, and Niche fibroblasts treated with rhCTGF with or without PD98059 or PAF-C16. All in vitro experiments are performed in three independent replicates. Adjacent, adjacent myometrium tissue of niche; Control, well-healed cesarean scar tissue; Niche, niche tissue; HUF, human uterine fibroblasts; siNC, NC-siRNA; siLRP1, LRP1-siRNA. Source data are provided as a Source Data file. Created in BioRender. He, C. (2025) https://BioRender.com/krpej6u --.

Article Snippet: Following transfection with LRP1 siRNA, NC siRNA, lrp1 siRNA or nc siRNA for 48 hours, the cells were subsequently incubated with rhCTGF (HY- P70106 , MCE), PD98059 (HY-12028, MCE), or C16-PAF (HY-108635, MCE) for 48 hours.

Techniques: Derivative Assay, Control, Recombinant, Western Blot, Expressing, Transfection, In Vitro

Journal: Nature Communications

Article Title: Role of CTGF-LRP1 in impaired healing of cesarean section incisions

doi: 10.1038/s41467-026-69747-9

Figure Lengend Snippet: a Experimental timeline for the establishment and treatment of the rat uterine scar model. b Schematic illustration and gross anatomical image of the rat uterine scar model. c H&E staining of uterine horns on days 14 and 30 post-surgery across sham, PBS, and rhctgf-treated groups ( n = 8 uterine horns per group). Red arrows indicate scar repair sites. Scale bars = 500 μm. d Immunohistochemical staining for α-smooth muscle actin (α-SMA) at incision sites on days 14 and 30. Red arrows mark the repaired myometrial regions. Scale bars = 500 μm. The regenerating myometrium originated from residual tissue flanking the incision and appeared thin, irregular, and fragmented. e Masson’s trichrome staining assessing collagen deposition at incision sites on days 14 and 30. Scale bars = 200 μm. Quantitative analysis of collagen fiber area was performed. f , g Efficiency of lrp1 knockdown in rat uterine fibroblasts assessed at the mRNA level. RT-qPCR and Western blot analyses show Collagen I and III expression in fibroblasts transfected with nc-siRNA or lrp1-siRNA and treated with rhctgf. Data are shown as mean ± SEM. The p values were calculated using a two-tailed unpaired Student’s t -test. Source data are provided as a Source Data file. Created in BioRender. He, C. (2025) https://BioRender.com/krpej6u .

Article Snippet: Following transfection with LRP1 siRNA, NC siRNA, lrp1 siRNA or nc siRNA for 48 hours, the cells were subsequently incubated with rhCTGF (HY- P70106 , MCE), PD98059 (HY-12028, MCE), or C16-PAF (HY-108635, MCE) for 48 hours.

Techniques: Staining, Immunohistochemical staining, Knockdown, Quantitative RT-PCR, Western Blot, Expressing, Transfection, Two Tailed Test